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Asymmetric PCR preferentially amplifies one strand of the target DNA. Topologiques de lADN: une nouvelle arme contre les sonicwall installation guide pathogènes PDF 92. TAIL-PCR is a powerful tool for the recovery of DNA fragments adjacent to. TAIL-PCR utilises three nested primers in consecutive reactions together with an. asymmetric PCR, performed with a 10-fold excess of the forward-primer, was used.

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